The Catecholase-Catalyzed Reaction
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Catecholase-Catalyzed Reaction
Submitted by:
Katrina R. Mitchell
Instructor:
Dr. Diomede Buzingo
Biology Lab
30, September 2012
The Catecholase-Catalyzed Reaction
Introduction
To study how various factors affect a particular enzyme,you will need to be able to measure either the appearance of substrate or the appearance of product. We can use color to visualize product formation, you,ll need a means of measuring how much color change happens during the reaction.
Materials
4 test tubes
potato extract
distilled water
parafilm
pipet
catechol
spectrophotometer
Observation
We cleaned the test tubes and then begun to add the reaction mixtures in one by one. We begun with distilled water adding 5 mL to test tube B, and then 3 mL to the first test tube, 3.5 mL to the second, and 4 mL to the third. Next, we added 2 mL of catechol to test tubes one, two, and three. Then, added 1 mL of potato extract to test tubes B and one, 0.5 mL to test tube two, and 0 mL to test tube three. Put parafilm over the top to shake up the reaction mixtures.
Question
Will the spectrophotometer extract the catecholase (potato extract)?
Is the color intensity going to get lower when their is less catecholase?
Hypothesis
My observation is that the spectrophotometer will extract the light of the catecholase. The less potato extract their is the less the light will be. Test tube B is used for zeroing the spectrophotometer when you put in each test tube.
Result
My hypothesis was right and the results the test tubes were:
Test tube one result was:
.65 of absorbance/color intensity
Test tube two result was:
.33 of absorbance/color intensity
Test tube three result was:
0 of absorbance/color intensity