Essay About Romy Sacks Partners Names And Ml0.02Urea Std

Virtual Nitrogen Balance Practical
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Name: Romy Sacks Partners names: Erin Pratt, Laura Prac date: 1/09/15 Results used: Raw dataIntroduction: The aim of the practical session was to ensure the understanding nitrogen balance. Also, it aimed to identify the roles of urea, uric acid and creatinine. Moreover, the practical intended to show the influences of varying levels on protein intake on excretion.Methods: Refer to instructionsVIRTUAL NITROGEN BALANCE PRACTICAL        Tube12345Urea std (0.25 mg/ml) ml0.02Urea std (0.5 mg/ml) ml0.02Urea std (0.75 mg/ml) ml0.02Urea std (1 mg/ml) ml0.02Water  (ml)0.02Urease 1 mg/ml (ml)0.10.10.10.10.1Phenol solution  (ml)2.52.52.52.52.5Hypochlorite solution (ml)2.52.52.52.52.5Absorbance 546 nm0.0060.1710.3590.5350.696Urea (μg)05101520Tube67891011121314151617Sample – high (ml)0.020.02Sample – medium  (ml)0.020.02Sample – low  (ml)0.020.02Sample – X  (ml)0.020.02Sample –Y  (ml)0.020.02 Sample – Z  (ml)0.020.02Urease 1 mg/ml (ml)0.10.10.10.10.10.10.10.10.10.10.10.1Phenol (120 mM) (ml)2.52.52.52.52.52.52.52.52.52.52.52.5Hypochlorite solution (ml)2.52.52.52.52.52.52.52.52.52.52.52.5Absorbance 546 nm0.6900.6770.2710.3100.1000.1090.3200.3140.1040.1030.2400.235Urea (μg)[pic 1]           Line of best fit: y = 0.03488x + 0.0046Where y= absorption (546nm) and x= urea (µg) SampleAbsorbanceµg/20µlAverage µg/20µlUndiluted µg/20µlX 20µg/mlX 50Mg/mL/ 1000Mg/24hr (2L)X 2000g/24hr/ 1000Nitrogen g/24hrX 0.467High0.69019.719.53901950019.5390003918.20.67719.3Med0.2176.17.4514974507.451490014.96.950.3108.8Low0.1002.72.855728502.8557005.72.70.1093.0X0.3209.048.96179.1289568.951791217.98.40.3148.87Y0.1042.852.8458.628402.8456805.682.650.1032.82Z0.2406.756.68113.666806.681336013.366.240.2356.61Nitrogen in urea sample: CH4N20N = 2 X 14 = 28Molecular weight urea = 60(28/60) X 100 = 46.7The absorbance values obtained for the creatinine assay: ABSORBANCE VALUES FOR STANDARD CURVE: Tube 1 2 3 4 5 6 Creatinine std (100 ⎧g/ml ) (ml) 0 0.2 0.4 0.6 0.8 1.0 Distilled water 1.0 0.8 0.6 0.4 0.2 0 Alkaline picrate (ml) 2.0 2.0 2.0 2.0 2.0 2.0 Distilled water (ml) 7.0 7.0 7.0 7.0 7.0 7.0 Absorbance 520 nm 0 0.14 0.261 0.392 0.512 0.616 Creatinine (⎧g) 0 20 40 60 80 100 ABSORBANCE VALUES FOR ALL OTHER SAMPLES: