Biology
Essay Preview: Biology
Report this essay
SHALINI SUNDRAMURTHI CHELLIAH 27208664PRACTICAL 1 Table 1: Experimental protocol for construction of the protein standard curveTUBE123456Buffer (ml)1.00.80.60.40.20.0BSA Protein solution(10mg ml-1) (ml)0.000.20.40.60.810.0Biuret reagent (ml)4.04.04.04.04.04.0Total volume (ml)5.05.05.05.05.05.0Final protein concentration (mg ml-1)0.02.04.06.08.010.0Absorbance0.00.080.180.270.350.45Table2: Experimental protocol for determining the protein concentration of silverbeet grown in the sun and shade
SunShadeColumns for modified procedure(s)ProcedureProtein solution (ml)1.01.00.250.25Biuret reagent (ml)4.04.00.750.75Absorbance1.050.870.200.15Protein concentration derivedfrom standard curve (mg ml-1)[pic 1][pic 2][pic 3][pic 4]4.453.35Protein concentration of original,Unmodified solution (mg ml-1)[pic 5][pic 6][pic 7][pic 8]8.0013.40SHALINI SUNDRAMURTHI CHELLIAH27208664PART 1F1.[pic 9]Figure1. Determination of the protein concentration of unknown solutions using standard curveLine of best fit should be drawn. This is because all the residual plots are along the line of best fit.SHALINI SUNDRAMURTHI CHELLIAH