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Quenching of FluorescenceEssay title: Quenching of FluorescenceQuenching of Fluorescence:Use of the Stern-Volmer EquationIntroductionExcitation of some chemical compounds when radiated with visible or UV light results in electronic transitions to higher energy levels. The extent to which light of various wavelengths absorbed constitutes the absorption spectrum of the compound. The spacing between levels in the two.