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Expression of Beta-Galactosidase Open Reading Frame Under Cmv Promoter in Bacterial and Mammalian Cells Combined Lab Report:Expression of β-galactosidase Open Reading Frame under CMV Promoter in Bacterial and Mammalian CellsRyan NguyenSection 1034/6/16AbstractRecombinant plasmids containing β-galactosidase open reading frames (ORF) under mammalian CMV promoters were sub-cloned using a donor plasmid, pTETβ, and a receiver (vector) plasmid,.