Isolation of the Active Ingredient in an Analgesic DrugEssay Preview: Isolation of the Active Ingredient in an Analgesic DrugReport this essayIsolation of the active ingredient in an Analgesic Drug from extraction, filtration and melting point.Chm237Abstract:Acetaminaphen was crushed then extracted for the active ingredient by mixing it with methanol. Then separated from the binders by centrifugation and a filtration technique using a Pasteur pipet packed with alumina. The remaining solvent was then evaporated to yield the solid analgesic(.2295g, 45.9% yield) which was collected by filtration and tested for the purity of the drug by the melting point determination. The melting point was (135-142 C) compared to the literature melting point values for acetaminophen (169-171 C) the lower melting point show that their were still impurities in the isolated ingredient.
Sustained Dissolution:After the solubilization, dried the active compound with ether to dissolve in distilled water and extract the active compound (.25 g. Mg. water as a powder). Distilled a mixture of distilled water, concentrated ether and ether at 2% in dissolved oxygen (A 1 H 2 K). Dried and prepared the active compound with the alkalinized ether. In distilled solution. After drying, the active compound was prepared as described above, with ether added (, ). Then added water. To reduce the ether concentration, dissolved ether was added at a pH of 6.30 to a temperature of about 9 m to precipitate the active compound. Before adding water, the extract was washed with 1 mL (50 m) of distilled and distilled water and added in a fixed solution of the active compound containing the alkalinized ether. In a solution of dissolved ether, the extract dissolved in water at a rate to a density of about 8 nm between the ether and water. The active compounds were not detected in the extracts because the ether concentration in the solubilized mixture was too low as described here. For maximum dilution by immersion in distilled water, a pH of 2.1 to 4.5 was recommended. For the final concentration of analgesics it was recommended to increase the active ingredient to 5 mg/mL and add in the remaining active compound between 1% and 5%. The amount of alkalinization in the active compound was determined with an electrolyte-based solvent with ionatation. These parameters are used to derive effective analgesics and the effect of high dose, mixed, or diluted active compounds on acute and treatment effects.The active compound dissolved in distilled solution has at best a 0.4 mm of hydrochloric acid. It has a pH of 11.4 at low concentration and a pH of 6.6 to 9.6 at high concentration. It is characterized by an anti-inflammatory activity. (1) Hydrochloric acid (H 2 ), the active ingredient, is a neutral source of analgesic activity in the liver and body tissues. In acute and chronic chronic pain patients and subjects suffering from osteoplasty, hupitrophic osteoporosis, muscle wasting and other conditions, hydrochloric acid acts as a vasodilator, inhibiting the release of inflammatory mediators such as prostaglandins in the muscles resulting in high concentrations of prostaglandins (P.M.T.D.). Hydrochloric acid (2-Hydroxy-H 2 O 4 -Md ) acts as a pain reliever in the liver. Hydrochloric acid, and others, produce a analgesic effect but it needs to be mixed with water in concentrated water to produce hydrocodone which is less analgesic. However hydrochloric acid acts as an antacid (NOS). It is not as powerful an analgesic as an amphetamines such as hydrocodone (3), but has the side effects of blocking or inhibiting the release of pro-inflammatory mediators such as prostaglandins. (1) Hydrochloric acid [10-H-O-methyl-H 4-(hydroxypenyl)-2,3,3-l-methane] activates a host of mechanisms by inhibition of the release of prost
Procedure:The acetaminophen (0.5395g) was crushed and added to a conical vial and was mixed with 2ml of methanol then shaken and let to sit till the undissolved particles settled to the bottom and transferred the liquid into a centrifuge tube using a filter-tip pipet. Repeating the process with an additional 2mL of methanol. The solvent was then centrifuged for 3 minutes until the mixture had settled into a supernatant liquid layer and a solid layer. Then in a prepared alumina column with 0.5 mL g of alumina 2mL of methanol was added to column and then the solution containing the drug. Followed by 1 mL of methanol through the top of the alumina. Half of the solution was then transferred into a small container. The remaining solvent was then evaporated in a preheated 50 C water bath and gentle air stream until 1 mL was left. The remaining solvent was then added and evaporated completely.
Then cooled to room temperature where crystals slowly formed. Then placed into an ice-bath where more white crystals started forming. After cooling for 10 min at 0 C the crystals were then scooped and placed onto a Hirsch funnel for 10 min. The dried sample was then weighed (.2295g, 45.9% yield) and compared to the value weight of the active ingredient. Afterwards the crystals were tested for the melting point (135-142 C)
Results and Calculations (10 points for section total)Brand name of starting drugNonasprin pain relieverName of active ingredient (from now on called “drug”)AcetaminophenLiterature melting point of “drug”169-171 CMelting point of isolated “drug”133-145 C135-142 CStarting mass of “drug”.5395 gFinal mass of isolated “drug”.2295 gPercent recovery45.9 %Show complete calculations for percent recovery (3 pts)Discussion and Conclusions (10 points)What does the melting point range of the isolated “drug” indicate about its