Organic Solvent Stress on Membrane
Essay title: Organic Solvent Stress on Membrane
Beet Cell Lab
Purpose: The purpose of this Lab was to see how much pressure or stress the beet tissue would hold.
Hypothesis: I hypothesize that the higher % of the solvent that the higher the colour intensity will be shown on the ph colour scale. The lower the solvent then the less colour shown.
Materials:
*Test Tubes (4)
*Cork Borer
*Beets
*Water
*1% Acetone
*50% Acetone
*1% Methanol
*50% Methanol
Procedure:
*Cut 4 uniform cylinders of beet using a cork borer with a 5-mm inside diameter. Trim each cylinder to 15-mm in length.
*Place these cylinders of beet tissues in a beaker and run tap water over then for 2 minutes to wash betacyanin from the injured cells on the surface.
*Discard the slightly coloured solution, and place the 4 beets sections into 4 dry test tubes.
*Keep all beets at room temperature
for 20 minutes and shake them occasionally. After 20 minutes, remove and discard the beet sections and observe the extent of membrane injury according to the amount of betacyanin that diffused into the organic solvent.
*Quantify the relative colour of each solution between 0 (colourless) and 10(darkest red).
Record your data in the table. Graph relative colour versus organic-solvent concentration according to a demonstration graph provided by your instructor.
Observations:
Tube Number
Treatment
Colour intensity
1% Acetone
50% Actetone
1% Methanol
50% Methanol
2a)The results my group got for part b which was the colour intensity of betacyaninun leaked