Biochemistry Lab ReportEssay Preview: Biochemistry Lab ReportReport this essayResult:Table of absorbance reading at 510 nm with different volume of banana extract and 3ml of DNS reagentTest tubeVolume of banana extract (ml)Absorbance reading at 510 nmA12.207B22.302Table of absorbance reading at 510 nm with different glucose concentration and 3ml of DNS reagentTube no.Concentration of glucose (mg/ml)Absorbance reading at 510 nm10020.251.05230.51.721412.181522.270652.3367102.371 [pic 1]Figure of the absorbance at 510 nm against the concentration of glucose(mg/ml)Calculation
To calculate the amount of glucose in the banana sample, we need to add the two absorbance readings obtained then average it, that is(2.207+2.302)÷2=2.25By plotting the value of 2.2545 in the standard curve, we found out the concentration of glucose present in the banana sample is 1.3 (mg/ml).QuestionWhy Rochelle salt solution required to be added in while the test tubes still warm?Why the test tube need to be cooled to room temperature before absorbance reading is to be taken?This is because we need to wait the stabilization process of the colour formed is fully completed and stable as the colour intensity will affect the absorbance reading.Why it is necessary to neutralize the sample to pH 7.0?DiscussionIn this experiment, we used the Dinitrosalicylic Acid method to estimate the amount of glucose in the banana sample. We need to prepare a Glucose Standard curve to determine the amount of glucose present in the banana sample.
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